This is a journal on genetics and genetic engineering. This volume 4, issue 1 (2020) contains original research articles on the construction of human neuromuscular disease-related gene site-specific mutant cell line by Cas9 Mutation System, mining of related genes with high efficiency of phosphorus utilization based on transcriptome sequencing in soybean as well as the transcriptome sequencing of solanum nigrum linn reveals the genes related to the biosynthesis of flavonoids.